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However, repetition of these experiments in the SC formulation employed in other experiments confirmed that the effects of caloric restriction were the same in both formulations (not shown).
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All shear stress-time curves obtained from each formulation (not shown) were fitted by non-linear regression as described above, and the half time and final gel elasticity were plotted against the fraction of sodium alginate in the formulations.
It was found that animals rapidly lost righting reflex (within 0.5 min of dose administration) following administration of both of propofol formulations (data not shown).
Among all of the tested formulations (results not shown), only the combination of 3 g/l malic acid, 0.3 g/l citric acid, and 3 g/l tartaric acid allowed S288C to complete synthetic must fermentation up to must dryness (Figure 2).
Differences in vital signs and other laboratory evaluations were not significant between formulations (data not shown).
Similar results were obtained with other CAP formulations (data not shown).
Cell growth and proliferation were comparable in the Advanced media (1% FBS) and RPMI (5 10% FBS) formulations (data not shown).
Porphyrins released to media are about 30 35% of the amount retained intracellularly for all the concentrations of ALA and He ALA tested and for both formulations (data not shown).
Our findings indicate that even at high dosage (1.0 μg) of inactivated EV71 virion, the cytokine-secreting responses such as IFN- γ/IL-4-secreting cells (ELISPOT) and the IFN- γ/IL-4 concentrations in the splenocyte supernatants (ELISA) were found to be at the undetectable level regardless of the adjuvant formulations (data not shown).
The addition of the fluorochrome did not affect the immunogenicity of the formulation (data not shown).
However, data obtained from clinical trials with a tetravalent vaccine formulation have not shown the predicted response, as immune imbalance or reactogenicity occurred for certain DEN serotypes [6], [7].
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