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The formulation was stored at ambient temperature for further use.
Optimized CC solid NE formulation was stored at 40 °C and maintained at 75±5%5% relative humidity (RH) for 3 months (Memmert oven, W. Germany) and examined for particle size, polydispersity index, zeta potential and in vitro drug dissolution on days 0 and 90.
Each formulation was stored at 4°C.
Briefly, 120 mg of PEG-b-PLACL, 0.8 mL of aqueous solution (internal aqueous solution), and 1.1 mL of oily solution consisting of squalene (Sigma-Aldrich, Steinheim, Germany) and Span®85 (85/15 v/v) were emulsified using Polytron®PT 3100 homogeniser (Kinematica AG, Switzerland) under 6,000 rpm for 5 min. The emulsified PELC formulation was stored at 4°C until use.
The formulation was stored at 2 8 °C for a period of 3 mo and its stability was investigated by assessing the color and appearance, the pH, the protein content by the o-phthaldialdehyde (OPA) assay, and the identity by SDS-PAGE followed by Coomassie Blue staining.
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Studying The effect of the temperature on the long term stability of formulation were stored at 4 ± 2, 25 ± 2 and 45 ± 2 °C for 90 days.
Then, all formulations were stored at 4 °C until required (Lee et al. 2006).
All formulations were stored at room temperature and protected from light (amber glass flasks).
Dose formulations were stored frozen up to 7 days at −72°C to −76°C until the day of use.
The results of the population dynamics of Trichoderma spp. in extracts of C. vulgaris, when the liquid formulations were stored at 7 °C, showed that there was a slow and progressive decline of the antagonist populations in the bioformulations from 120 DAS to 180, 240, 300, and 360 DAS.
Both the test and reference formulations were stored at 4°C.
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