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Watson, D. S., Endsley, A. N. & Huang, L. Design considerations for liposomal vaccines: Influence of formulation parameters on antibody and cell-mediated immune responses to liposome associated antigens.
The formulation parameters modified the intrinsic properties of chitosan.
Initial in vitro experiments were conducted to optimize formulation parameters prior to transdermal delivery in rats.
Effect of formulation parameters such as polymer concentration and cross-linker concentration has also been evaluated.
Also, using experimental designs, statistical significance of the formulation parameters influence was estimated.
DoE regression analysis showed that all formulation parameters affect the dependent variables significantly.
Different formulation parameters can be varied to adjust desired drug release kinetics.
A fractional factorial experimental design was implemented to screen six formulation parameters.
A quality-by-design (QbD) approach was adopted to define the optimal formulation parameters.
Box-Behnken experimental design was used to statistically optimize the formulation parameters.
A Box-Behnken design (BBD) was used to optimize the critical process and formulation parameters.
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