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Exacerbated by the large number of available choices for detection chemistry, enzyme formulation and instrumentation, combined with a paucity of performance benchmarks, it is not surprising that a large number of studies have expressed concerns about the general efficacy of real-time qPCR [13], [14], [15], [16], [17], [18], [19], [20], [21], [22], [23].
The relevancy of this to real-time qPCR is particularly evident in view of the large number of available choices for detection chemistry, enzyme formulation and instrumentation, all of which generates an enormous number of options to choose from.
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The plethora of choices currently available for detection chemistry, enzyme formulation, cycling regime and instrumentation provide many compelling examples.
Bagajewicz, Design and retrofit of sensor networks in process plants, AIChE J. 43 (1997) 2300 2306; M. Bagajewicz, E. Cabrera, New MILP formulation for instrumentation network design and upgrade, AIChE J. 48 (2002) 2271 2282] have been proposed as a criteria for optimally locating sensors.
Yet knowledge and instrumentation had proved grossly insufficient.
Methods and instrumentation.
and instrumentation readings within the 3D settings.
To meet these objectives specific strategies and instrumentation are required.
Figure 2 shows the test set-up and instrumentation.
Authors thank Anna University Trichy, Chennai for technical help and instrumentation usage.
Figure 5 shows the test setup and instrumentation.
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