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In addition, to explore the influence of conductivity of the medium, we have used low conductive formulated medium with conductivity of 81.4 μS/cm.
If such a formulated medium is used for perfusion culture outside a CO2 incubator, the pH will shift from the physiological range to much more alkaline values due to the low content of CO2 (0,3%) in atmospheric air.
Our formulated medium from cheap, easy accessible ingredients performed comparably to commercially available RPMI and LSSM.
In this study, a co-culture model was used in which endothelial cells are co-cultured with fibroblasts in a specially formulated medium.
The final formulated medium, excluding glucose and vitamin solution supplementation, is adjusted to pH 5.0 with 2 M NaOH and heat sterilized.
Reprogramming of mouse embryo fibroblasts is initiated but then aborted, and cells are put into a newly formulated medium that allows for the production of (in this case) cardiac myocytes [ 41].
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Significant improvement in biomass and lipid production was obtained using newly formulated fertilizer medium over f/2 medium.
A significant improvement in biomass, lipid and eicosapentaenoic acid productivity is obtained from the optimally formulated fertilizer medium using genetic algorithm over response surface method based fertilizer medium and standard culture medium.
These data demonstrate that the inventive modified culture system using the newly formulated GBGM® medium results in more than 4-log expansion of pure human NK cells from freshly selected CD34+ UCB cells.
The human monocyte cell line U937 was cultured in ATCC formulated RPMI-1640 medium (catalog number 30-2001), dintorentiated into macrophages by PMA treatment at 50 ng/mL.
The human monocyte cell line U937 was grown in ATCC formulated RPMI-1640 medium (catalog number 30-2001) containing 10% fetal bovine serum (Atlanta Biologicals), subcultured in GIBCO RPMI-1640 (Catalog number 11879-020, supplemented with 5 mM D-glucose), and differentiated into macrophages by phorbol 1 2-myristate 13-acetate (PMA, 50 ng/mL) treatment.
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