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The investigation of the effect of formulation variables on nano-spanlastic characteristics and selection of the optimum formula were performed using Design-Expert® software.
Measurement of serum specific IgE against CMP (alpha-lactalbumin, ALA; beta-lactoglobulin, BLG; and casein); SPTs with whole milk, CMP, placebo formula (Neocate, Nutricia, Milan, Italy) and study formula (Sineall, Humana, Milan, Italy); and APTs with whole milk, placebo and study formula, were performed in all patients.
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Final optimization of formula was performed by response surface methodology using a central composite design.
If it was suspected that the formula caused symptoms, a double-blind, placebo-controlled challenge (DBPCFC) with the formula was performed.
Detailed analysis of this formula is performed for the chronocoulometric response of fractal and nonfractal electrodes using an approximate power-law and Gaussian power spectra, respectively.
Adjustment of the incidence formula was performed according to Hargrove et al. [ 13].
Complete blood cell count with leukocyte formula was performed using an automated cell counter (Cell Dyn 3500, Abbott Laboratories, Abbott Park, IL, USA).
Then another TACE with the same formula was performed to enhance local control of the intrahepatic lesions, with sorafenib continued.
The determination of the most probable elemental formula is performed in both MS and MS/MS modes, for molecular adducts and fragment ions as well.
The complete blood cell count with leukocyte formula was performed using an automated cell counter (Cell Dyn 3500, Abbott Laboratories, Abbott Park, Illinois, USA).
Thus, a further analysis using CLUMP, a computer method similar to Chi-square analysis that uses a Monte-Carlo style probabilities rather than a set probability formula was performed.
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