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The discovery and characterization of multiple, functionally distinct forms of Mediator characterized by the presence or absence of the Cdk8 kinase module have led to new insights into how Mediator functions in both Pol II transcription activation and repression.
The Cdk8 kinase module (CKM), among other functions regulates the interconversion between active and repressive forms of Mediator (Poss et al., 2013).
In silico and genome-wide expression analysis of plant Mediator subunit genes under stress conditions revealed differential transcript abundance and alternative forms of Mediator complexes in different cell types and developmental stages (Mathur et al. 2011).
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Inflammatory cells release various forms of mediators into the nasal mucosa, ranging from symptom-producing substances to pure cytokines that perpetuate chronic inflammation and symptoms.
The ratio of one-electron transfer (ET) to a two-electron process (SN2-like nucleophilic attack of the reduced form of mediator on the chlorosilane, with k2 ≅ 102-108 M−1 s−1) inverselyely related to the steric availability of Si for nucleophilic displacement reactions.
Apart from MED12, all of the components identified belong to the active form of Mediator.
The larger form of Mediator (L Mediator), including CDK8 submodule, associates with decreased gene transcription (Knuesel et al, 2009a).
The complex is not only a functionally relevant form of Mediator, but is also more soluble than free Mediator under the conditions used for lysine directed cross-linking.
The model equations describe dynamic diffusion and reaction of the involved species (oxygen, oxidized/reduced forms of the mediator - Prussian Blue/Prussian White).
The constant core and the variably associated proteins are considered to be components of the active forms of the Mediator complex.
The oxidized form of the mediator (II) reacts with secondary amines such as methamphetamine (MAMP) by 1,4-addition resulting in the reduced form of the MAMP-mediator adduct (III).
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