Exact(1)
The purified fragment was digested with NcoI and NotI and ligated (using T4 DNA ligase NEB #002025) at the corresponding sites of pET-28b, forming the constructed plasmid pET28b-kIspS (Fig. 1).
Similar(59)
The work carried out contributes to meeting the growing need for cross-cultural research into the dimensions forming the construct of authentic leadership.
The first two short consensus repeats (SCR) of complement receptor 2 (CR2) were cloned into the pET24a bacterial expression vector with the addition of a C-terminal spacer and a HexaHistidine tag (His-tag), forming the construct rCR2.
Internal consistency was not examined because the FI is a formative model, i.e., the items form the construct together and therefore do not need to be correlated [ 39].
Each aspect is a unique part of the provided care and together they form the construct 'quality of care'.
The IMS-S starts with 5 items that measure concrete examplars of satisfaction, to enhance the comprehensibility of the global items, which are utilized to form the construct.
The SMAS-S consists of six three-item subscales (taking initiative, investment behaviour, variety, multifunctionality, self-efficacy and positive frame of mind), which reflect core abilities to form the construct of self-management of well-being [ 24].
When the raters are not able to distinguish the vocabs and disagree to the list coming together to form the constructs, α would be 0.
One group used monoaxial screws linked with pre-bent rods of various angles to form the spinal constructs, and the other group adapted polyaxial screws to form the constructs.
This fragment was cloned into a pGL3-Promoter luciferase reporter vector upstream of a minimal SV-40 promoter, forming the pGL3-PRAP1 construct.
Given that the usual reliability and validity criteria do not apply to a formative construct, it must first be verified that there is no multicollinearity among the indicators forming this construct [ 70].
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