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Phospho-Erk1/2 proteins in this cell line were restricted to the cell membrane in areas of cell-to-cell contact at the periphery of large multicellular clusters (see Additional File 4, Figure S2), and were not seen in individualized cells or in cells forming smaller clusters, suggesting activation was probably mediated by intercellular signaling.
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These cells appeared to grow by cell division on the surface, forming small clusters of cells (Figure 1c).
This could be caused by a discontinuous character of the gold layer possibly forming small clusters of gold which are dissolved faster [47].
Some of these small cells proliferated, forming small clusters of cells that grew to a diameter of up to 15 μm.
It is also consistent with the higher number of keratin-positive single-tumour epithelial cells and higher number of tumour cells forming small clusters (tumour budding) at the invasion front in HCT116 SC+ORT primary tumours.
Dr Lumley noticed that, if he stopped the heat treatment at just the right point, he could prevent the precipitates from growing too large and encourage them to form smaller clusters with a finer distribution.
Cells so treated migrated normally, but formed smaller clusters that failed to aggregate further.
To determine if any of the PSGs interact with each other and form smaller clusters of adaptive sub-networks, we queried the same database with the lists of PSGs from each lineage.
IRGs that have a low number of partners (small downward peak in Figure 3, left panels for average node degrees) and belong to many triplets (large downward peaks in Figure 3, right panels for clustering coefficients) are thought to have highly specific interactions to form small clusters.
The five-petaled flowers, commonly 2 3 cm (about 1 inch) in diameter, are solitary or form small clusters.
We find that the early TM elements do not segregate together while the late elements tend to accumulate to form small clusters in dilute W alloys.
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