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After trypsin digestion, 13 peptides were identified upstream the formerly annotated starting M residue, confirming that the XopD protein sequence annotated in the public databases is not complete.
We note that the Rmet_ 6291 gene, formerly annotated as pilQ [10], now is annotated as pilB because its product shows no aa sequence similarity to the PilQ usher protein encoded in the pilMNOPQ locus on CHR1 (Rmet_3268 to Rmet_3272); rather, it probably acts as an ATPase.
In fact, LmjF32.2740 is homologous to eukaryotic arsenate reductases from S. cerevisiae, ScACR2, and Arabidopsis, AtACR2 (formerly annotated as cdc25).
Two proteins with SOUL heme-binding domains were among SAK1-dependent up-regulated genes (SOUL2 and Cre06.g299700.t1.1, formerly annotated as SOUL1 in v4).
In Methylosinus trichosporium OB3b, and one other operon detected (Methylobacterium sp. B34), MbnB is split into two ORFs, formerly annotated as MettrDRAFT_3894 and MettrDRAFT_3895, but reannotated as one entity (MettrDRAFT_3422) in a recently assembled genome build available on IMG [ 23].
Another function that was notable among up-regulated genes, although they were not grouped to a common functional class by MapMan, were two genes coding for SOUL heme-binding domain proteins that were SAK1-dependent (SOUL2 and Cre06.g299700.t1.1, formerly annotated as SOUL1).
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Recently, the genome of H. irregulare (formerly H. annosum North American P-type) was sequenced and partially annotated (Olson et al. 2012).
vesicatoria {Xav, a pathogen of tomato and pepper plants; formerly X. campestris pv vesicatoria [ 28]}, where it is annotated as a putative carbohydrate translocase (YP_365447.1; identity 76% and similarity 87%) [ 29] and in Yersinia bercovieri ATCC43970, where it is annotated as a hypothetical protein [ZP_00820359.1; identity 36% and similarity 54%).
The Brassicaceae (formerly Cruciferae) family is an exemplary model for studying polyploidy events because the well annotated Arabidopsis thaliana (A. thaliana) genome exists as a reference [ 10], with its well supported three rounds of WGD (At-α, At-β, and At-γ) [ 11].
The principle changes made are an updated and enlarged survey of human Dux family sequences and genes, inclusion of the NANOGNB gene (formerly C14) and its pseudogenes, identification of four Cphx family loci and inclusion of the newly annotated locus LOC647589.
NCU03459 is annotated as encoding an ubiquitin binding associated (UBA) protein and has sequence similarity to Dsc2p (formerly YOL073C) in S. cerevisiae and Dsc2 (formerly Ubc14) in S. pombe, both of which function in association with a Golgi E3 ligase complex [ 31, 32].
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