The former variant is shown to require the least accommodation strain within the parent grain and to have the greatest potential for growth.
Whereas the former variant of reduction did not attract much attention (by Nagel and others), the latter has been a subject of intense discussion since Nagel introduced it in 1949.
For future experiments, the former variant (CFP-EPAC-cp173Venus) combines a good FRET span with small size.
Since the NN-tanglegram heuristic performs similarly on trees when using the splits-based distance H or the shortest path distance matrix H′, we will present the results only for the former variant.
Although all four studied missense variations (R96G, I261M, E276K, and Y374N) were predicted to be damaging by SIFT and/or PolyPhen, only the former variant was found to be deleterious, but the latter three variants were found to have nearly neutral effects on pol ι function in our study, indicating the substantial false-positive nature of in silico prediction.
Since the NN-tanglegram heuristic performs a lot better on networks when using the shortest path distance matrix H′ rather than the splits-based distance H [see Figure 6(b), NN-tanglegram+1S NN-tanglegram+1S_ C +1 S], we will discuss the results only for the former vsriant.
For the former variant, the EGFP coding sequence was amplified using the primers RaS42 (5′-C GATGTC ATGTGTG AGC AAG GGC GAG-3′) and RaS43 (5′-TGGATTTCTG GCTTCCGTACAG GTA CTC CTC GTC CAT G-3′), which introduce an EcoRI and a NotI site before the initiation codon or the stop codon, respectively.
The former two variants were found once in the 116 cases, while the V945I variant was present in 2 cases.
Some dialects of the Netherlands are closer in linguistic properties to dialects of neighbouring Germany than they are to dialects spoken on the Dutch coast, despite being grouped with the latter but not the former as variants of the same language, Dutch.
Therefore, the current proviral DNA can be considered a good mirror of the patient viral quasispecies, including both recent or former replication variants [ 28, 36].
