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It is therefore likely that the apparent EC50 of Ch or for α7 nAChRs is lower in synaptosomes than in electrophysiological preparations in view of the longer exposure time and higher efficacy of the agonist in the former preparation.
Membrane bound ADP-ribosyl cyclase activity has previously been described in several cell types including sea urchin egg and sperm homogenates [27] [29], [46], [51], the former preparation composed predominately of intracellular membranes.
The observed differences indicate the slightly smaller amount of non-specific binding and the stronger specific binding of the former preparation.
This difference between the native and expressed RoDH activities may be due to an averaging effect of isoenzymes in the former preparation, because at least one other RoDH has been detected in rat brain (Chai et al., 1995).
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Back home in Austria, the two women had watched all the movies about the former prison in preparation for their visit.
Mr. Klein sold the building years ago, and the current owners, Hines Interests of Houston, knocked out the dropped ceilings from his former office in preparation for a new tenant.
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And yet, with minimal preparation, the former world champion managed to get herself into good enough shape to return with her fastest time over 26.2 miles since she won in New York in 2007 in 2 23:09.
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CEO of Professional Science Editing for Scientists @ prosciediting.com