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Pro-angiogenic function was enhanced, as cells readily formed tubes.
Comparison of the experimental results and the properties of the flow formed tubes predicted using empirical relations shows that empirical relations can be used for predicting the properties of flow formed tubes with reasonable accuracy.
Similar to MS1 cells, HUVEC formed tubes, which contained both exogenous (Fig. 3D) and endogenous (Fig. 3E) laminins inside the lumen.
In addition, cultured ECs formed tubes on Matrigel-coated plates (BD Biosciences).
While the parental SVEC cell line underwent limited tubulogenesis, the vGPCR-expressing cells readily invaded the matrigel and formed tubes.
HMVECs formed tubes to Id1 at 10 nM, which was the peak concentration for HMVEC chemotactic activity.
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Further indicating their endothelial phenotype and function, cells formed tube-like structures on solidified Matrigel and showed migration on fibronectin surface in wound healing assays (Fig. 3E F).
On average, 18.7±4.8% of these islets formed tube-like structures protruding from the islets in the gel in the presence of 5 ng/ml bFGF.
EC formed tube-like structures, with the 10T1/2 cells associating with the endothelial abluminal surface and mimicking their association in vivo (Figure 10E).
Within 24 h, these stem cells formed tube-like structures after culturing on Matrigel).
In this model, new capillary vessels erupted from the tissue in matrigel, and new erupted endothelial cells grew from tissues in fibrinogen proliferated and formed tube.
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