Sentence examples for formation overnight from inspiring English sources

In order to test the effect of 5-iodoindole on persister formation, overnight cultures of E. coli BW25113 or S. aureus 6538 were diluted 1 1000 with 25 mL of LB and incubated at 37 °C with shaking at 250 rpm to a turbidity of 0.8 at 600 nm.

The contrast between the elongated phenotype of Inv rep cells after a shift to LB and a wild-type efficiency of colony formation overnight on LB plates suggest an early defect followed by a recovery.

rpoC* restored 100% overnight colony formation on LB in both Inv dinG mutants.

Because five generations occur during overnight plaque formation [ 19], co-infection level was manipulated every fifth generation and the lineages experienced roughly 300 generations of viral evolution [ 10].

In the overnight hours after formation, the storm was disorganized with a ragged and ill-defined center of circulation.

Rosette inhibition assays determine whether antibodies added to parasite culture at ring stage (before rosetting begins) and incubated overnight, prevent the formation of rosettes when the parasites mature to the pigmented trophozoite stage.

The bacteria were seeded with a confluent NIH 3T3 (mouse fibroblast) cell monolayer overnight for biofilm formation.

The blood was allowed to stand at room temperature for a couple of hours and then kept at 4°C for overnight to allow formation of a firm clot.

Soft-agar overlays containing 100 μl BCC liquid culture were allowed to solidify for ≥10 minutes at room temperature, spotted with 10 μl drops of high-titer JG068 stock, and assayed for clearing and/or plaque formation after incubation at 30°C overnight.

4-Phenyl-5 o -tolyloxymethyl-4 H -[1,2,4]triazole-3-thiol (6 d): To a solution of (4 a) o-tolyloxyacetic acid hydrazide (2.0 g, 11.095 mmol, 1.0 equiv) in EtOH (100 mL) was added phenyl isothiocyanate (1.356 mL, 11.095 mmol, 1.0 equiv), and the reaction mixture was heated under reflux for overnight, and the formation of precipitate was observed.

To this solution was added N,N-diisopropylbenzamide (0.205 g, 1 mmol) by using a solid addition tube and the mixture was stirred for 30 min. Cooling to −28 °C overnight led to formation of a crop of colourless crystals (0.41 g, 81%% yield).