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In order to further investigate the role of Hrs and the Hrs/Stam complex in FGFR signaling, we analyzed the effect of these mutations in the formation of projections by tracheal TCs, another cellular process which requires FGFR signaling.
The SipA mutant induced the formation of projections that contain actin at the base of the ruffle, but mainly villin in the apical part, exacerbating what was observed with wild-type (WT) Salmonella.
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Thus, there is likely to be an active sensing mechanism involved in the formation of multiple projections, rather than the projections emerging in random directions.
Projections were also absent from mid tailbud (Figure 6O,S) and late tailbud (Figure 6P,T) stage embryos, suggesting that formation of these projections is impaired in sna>Bix embryos, rather than being delayed in onset.
The formation of cytoplasmic projections, or pseudopodia, on the forward edge of the cell, pulling the cell along, is characteristic of the microscopic unicellular protozoans known as amoebas.
This research implicates Ste5p as a key player in the formation of multiple projections.
On the other hand, the formation of multiple projections induced by α-factor is sequential [8].
We found a genetic manipulation (AI-Ste5p sthat) that enhanced the formation of multiple projections.
The sst2Δ and ste2300Δ bar1Δ cells exhibited additional defects in the formation of mating projections in response to a gradient.
Transcriptional activation of mating-related genes, formation of mating projections, and fusion of two opposite mating type cells are involved in this process.
Transcriptional activation of mating-related genes, formation of mating projections, and fusion of the two opposite mating type cells are involved in this process.
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