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The optimum BHF of 1.62 MPa was determined which can avoid the formation of drawbacks of wrinkle and crack.
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This procedure is very promising but has the drawback of the formation of a buffer layer consisting of a graphene-like sheet, which is covalently bound to the substrate.
A drawback is the formation of by-products that inhibit enzymes and microorganisms in subsequent biocatalytic conversion steps.
Several works in the literature consider the formation of these inclusion bodies as a drawback of the expression system in E. coli, because they require further addition of a complex process of isolation and purification, in which proteins are denatured and refolded in vitro.
And there are other kinds of drawbacks.
Phytoremediation has its share of drawbacks.
But there are a number of drawbacks.
Jokes aside, there are a lot of drawbacks.
However, it has a couple of drawbacks.
However, immunohistochemistry has a number of drawbacks.
However, it carries a number of drawbacks.
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