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FD10GFs constructs formed better quality cartilage-like tissue than FD constructs in term of overall cartilaginous tissue formation, cells organization and extracellular matrix distribution in the specimens.
For analysis of ROS formation, cells (human macrophages or neutrophils) were preincubated with the peroxide-sensitive fluorescence dye 2′,7′-dichlorofluorescein-diacetate (1 μg/ml) for 10 min at 37 °C.
The implanted PDRN causes an angiogenesis phenomenon in neighboring soft tissue and induces [4, 5] new blood vessels to the implant site and differentiates [6 8] undifferentiated mesenchymal cells of the host into fibroblast and osteoblast, and then differentiated bone formation cells form new bones such as osteoid.
To analyse metastasis formation, cells were injected intravenously in SCID mice.
During the process of nematocyte formation, cells divide to form nests of nematoblasts connected by cytoplasmic bridges [48].
To study cell-matrix contact formation, cells were seeded onto plates coated with the extracellular matrix proteins fibronectin or collagen.
To determine SAFH formation, cells were cultured directly on glass cover slips and then fixed with 4% Para formaldehyde.
To determine the level of complex formation, cells were co-transfected with IκBα-ECFP (0.4 µg/well) and EYFP-relA (0.4 µg/well).
During blastocyst formation, cells in the inner cell mass (ICM) reactivate the paternal X chromosome whereas the trophectoderm and primitive endoderm retain their imprinted XCI.
To determine whether dynamin is involved in Trypanosoma cruzi entry and in phagolysosome formation, cells were infected with the three T. cruzi forms (trypomastigotes, epimastigotes and amastigotes) in the presence of dynasore at varying concentrations.
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One approach to such defects is to enhance the capacity of bone-formation cells.
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