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Tube formation assay demonstrates that ETV2 initiates morphological changes resembling endothelial process in vitro.
We used the standard colony formation assay to estimate the clonogenic cell survival.
For the indamine dye formation assay the coloring reagents were applied as an agarose overlay.
Biofilm formation assay was performed according to the method described previously (Boyd and O'Toole 2012).
The ability of cell proliferation was accessed by colony formation assay.
(A) Cartoon showing in vitro hESC-mouse blastocyst chimera formation assay.
(H) Colony formation assay with cells cultured for 48 h in suspension conditions.
For the tube formation assay, MSC-CM was then concentrated and stored as described.
The network formation assay showed that the induced hAF-MSCs formed partial networks.
Cell survival rates were measured by the colony formation assay.
Colony formation assay was performed as previously described [14].
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