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Supplementary Table S4 gives an example of a Cildb output in xls format, in this case a list of the 975 proteins of Danio rerio with orthologs in at least three different species identified as a ciliary protein with medium confidence (see definition in Supplementary Table S1).
To their great credit, they also effortlessly take back the XL format usually dominated by men.
LA-iMageS uses datasets in PerkinElmer Elan XL format (*.xl) as input, which is commonly generated by ICP MS instrument control software from PerkinElmer, such as Elan 6 × 00 or Elan DRC-e.
Elemental data is extracted from the input dataset in XL format, using the two optional configuration files if necessary.
As previously explained, LA-iMageS accepts ICP MS data in the PerkinElmer Elan XL format, where data are organized as rows and columns.
Since existing solutions are usually commercial or hard-to-use for average users, this work introduces LA-iMageS, an open-source, free-to-use multiplatform application for fast and automatic generation of high-quality elemental distribution bioimages from LA ICP MS data in the PerkinElmer Elan XL format, whose results can be directly exported to external applications for further analysis.
This is precisely the direction of this study, which proposes the LA-iMageS software to easily process all the data generated by LA ICP MS in the PerkinElmer Elan XL format, ensuring a fast processing workflow (data processing is done in few seconds after inserting the data) as well as the generation of elemental distribution images (with a quality comparable to those obtained through MatLab).
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