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FASTA format sequence for, in genetic domain original style, using the SEQRES ATOM PDB records.
The sffinfo command was used to extract fasta format (sequence data) and quality (quality scores) files from a raw.sff file with –s and –q options, respectively.
The FASTA format sequence of AKR1C2 was taken from UniProt[11] (accession number: P52895) and edited to reflect the amino acid change in the variant.
Six sequence files were generated in FASTQ format (sequence read plus quality information in Phred format); each file corresponded to the brain tissue from which the RNA originated.
Then the sequence was stored as a FASTA format sequence for further analysis.
Sequence files were generated in FASTQ format (sequence read plus quality information in Phred format).
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To minimize costs, it is recommend to use the 96-well plate format sequencing to confirm the introduction of the desired mutations.
FASTA format sequences are extracted from FASTQ files and those<400 base pairs (bp) in length (or<350 bp for the 18 S amplicon) or containing N's or homopolymer runs of>8 bp are removed using MOTHUR, (v1.34.1 20,21.
These are high definition (HD) YUV 4 2 0 format sequences downsampled to a resolution of 768 × 432 pixels.
In our experiment, thrdsmooth is set to 200 bytes for CIF format sequences coded with H.264 encoder and QP equal to 28.
The input of VERSE includes NGS reads (in FASTQ format) sequenced from a host, a reference host genome (in FASTA format), and a reference virus genome (FASTA format).
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