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The most common format for detection concurrent with unbiased amplification is the 5'-nuclease assay [ 1, 17].
Our study used a single-round, closed-tube, PCR format for detection, which has a high sensitivity and a greatly reduced risk for carryover contamination (14 ).
Owing to its potential for high throughput screening of large numbers of samples, we have also performed a preliminary evaluation of the commercial SNPstream genotyping system (GenomeLab, Beckman Coulter) that also utilises the "tag-array" primer extension strategy in a semi-automated 384-well microtiter plate format for detection of imbalanced allelic expression [ 30].
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The as-fabricated PEC sensor demonstrates many advantages such as wide linear range (5 pg mL−1 11.6 ng mL−1), low detection limit (1.67 pg mL−1, S/N = 3), and remarkably convenient, which provides a general format for chlorpyrifos detection in food and environment analysis.
It may also be a useful and convenient dipstick format for drug detection.
The implantable enzyme amperometric biosensor continues as the dominant in vivo format for the detection, monitoring and reporting of biochemical analytes related to a wide range of pathologies.
Under optimal conditions, the as-fabricated PEC sensor demonstrates many advantages such as low detection limit (0.1 μM, S/N = 3), wide linear range (0.01 ∼ 7.12 mM) and remarkably convenient, which provides a general format for glucose detection in blood serum.
The competitive inhibition format is found to be the most suitable and robust format for the detection of small organic molecules like mycotoxins.
MIRACLE processes each image into a zoomable format for visual detection of quality issues, such as clogged tips, scratches or uneven stainings.
The assays utilize a minimum steps for sample workup and can be run in a duplex format for the detection of neuronal ceroid lipofuscinoses or potentially multiplexed with other mass spectrometry-based assays for newborn screening of lysosomal storage disorders.
The amplified PCR products were tested using a probe hybridization with a cocktail of conservative probes recognizing at least 54 mucosal HPV genotypes in a microtitre plate format for the detection of HPV DNA.
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