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Experiments were designed in a triplicate format and cell proliferation rates were expressed as percentage proliferation compared to controls.
Images from the Zeiss ZVI files were converted into jpeg format and Cell Profiler software (http://www.cellprofiler.org/) was used to perform automatic identification and measurement of biological objects.
Migration assays were performed using a modified Transwell chamber (Corning, Inc). in a 24-well plate format and cell culture inserts containing filters with a pore size of either 3 μm (for neutrophilic granulocytes) or 5 μm (for PBMCs).
In converting this assay from a 24-well to a 384-well format, the effect of FUS knockdown on cell viability was reduced from ~40 to ~25%, perhaps because of the reduced plate format and cell plating.
In order to reveal the influence of different chemokines within the serum samples on inflammatory cells, PBMC migration assays were performed using a Transwell chamber (Corning Inc., Tewksbury, USA) in a 96-well plate format and cell culture inserts containing filters with a pore-size of 5 μm (Corning Inc., Tewksbury, USA).
Similar(55)
On the cell level, different cell formats and cell chemistries are discussed and the general battery pack design is highlighted.
Into cell B2, enter Factored and do Format Cells for cell range B2 B3 Border black bold outline, aligned center.
The data format and the cell-wise and marginal proportions are shown in Table 2.
Select cell range G1162 H1746 and Format Cell Fill Canary Yellow and Border Bold Dark Blue.
E3 103 and Format Cell Number Number Decimal Places 0. Format cell Fill yellow, border red bold outline.
Select cell N4 and Format cells Fill Yellow and Insert Name Define Selector to cell $N$4, with the Sheet Name and exclamation point preceding it, OK.
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