Sentence examples for formaldehyde were added from inspiring English sources
After centrifugation (1300 g, 5 minutes, 4°C) nuclei were carefully resuspended in 1 ml buffer A. 9 ml pre-warmed buffer A supplemented with 1.1% formaldehyde were added and the nuclei were cross-linked for 10 minutes at 37°C.
Then, 500 μl of PBS+ and 13.5 μl of 36.5% formaldehyde were added for crosslinking.
Just prior to fixation, 5 mL of 10%% aqueous formaldehyde were added.
In short, 250 µl 10% Triton X-100 and 500 µl 37% formaldehyde were added to 5 ml of cell suspension.
The protein was dialyzed against 10 mM HEPES, 150 mM NaCl, and 1 mM DTT buffer (pH 7.4); 0.25 mL of 1.6 mM borane dimethylamine complex [(CH3 2NH·BH3] and 0.5 mL of 1.6 mM C-labeled formaldehyde were added to 0.5 mL of 0.1 mM protein, and the reaction mixture was incubated while being stirred at 4 °C.
Because both geniposide and formaldehyde were added to SH-SY5Y cells at the same time, and it was shown that geniposide decreased cytotoxicity following formaldehyde treatment, we could not exclude the possibility that geniposide directly interacted with formaldehyde and then rescued the viability of cells.
For a typical synthesis, 1.0 g of F127 was dissolved in 20.0 g ethanol, and then 20.0 g of resol-ethanol solution containing 1.22 g of phenol and 0.78 g of formaldehyde was added.
At a word, 3T3-L1 cells were washed twice with phosphate-buffered saline (PBS) after removed the culture medium and were fixed in 10% formaldehyde at room temperature for 10 min, the 10% formaldehyde was discarded and new 10% formaldehyde was added for 1 h.
Formaldehyde was added to reach 0.0125% final concentration and the remaining insolubles removed by microfiltration.
