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Mouse lungs were collected and fixed in 10% formaldehyde for at least 24 h.
Isolated spinal cords were kept in 4% formaldehyde for at least 1 week, at 4 °C.
Specimens were fixed in 10%% formaldehyde for at least 24 h.
Sample fixation for tissue samples were done in 4% buffered formaldehyde for at least 1 week at 4 °C before shipping to the histology lab.
An opaque gel formed within 2 min, and the tissue surrogate was allowed to sit at room temperature in the presence of formaldehyde for at least 24 h to mimic typical tissue fixation methods.
The specimens were fixed in 4% formaldehyde for at least 7 days.
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Liver tissues from cats were fixed in 10% formaldehyde for overnight at 37°C.
Add 150µl fixing solution (PBS + 3.7% formaldehyde) for 15min at room temperature on the bench top (do not shake).
Single cell suspensions were fixed in 0.5% formaldehyde for 10 min at 37°C followed by 90% methanol at −20°C.
For ChIP-seq, sorted cells were crosslinked with 1% formaldehyde for 10 min at room temperature.
NES cells were washed with PBS and fixed with 4% formaldehyde for 10 min at RT.
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CEO of Professional Science Editing for Scientists @ prosciediting.com