The Rho-GTP form was isolated by pull-down assay (Pierce, Perbio, France) according to the manufacturer's instructions.
After carbonization the MgO was dissolved out using a diluted sulfuric acid and the carbons formed were isolated.
After 7-days incubation at 25°C, colonies formed were isolated as alliin-utilizing strains, and were independently inoculated onto the agar plates for evaluation of odorous compound production.
The Bass Strait forms were isolated from Tasmania but more recently and so their subspecific status was not maintained.
Midgut forms were isolated from infected flies and fixed with 4% formaldehyde in order to preserve fluorescence.
The cell monolayers were overlaid with 1% agar and the plaques that formed were isolated for further processing as detailed in a supplement.
In successful copulation the hybrid zygotes that formed were isolated via negative phototaxis and cultured up to their reproductive stage, the sporophyte.
Mutant AOX forms were isolated and purified, and some of their biochemical properties were studied.
The red-brown crystals formed were isolated by filtration and dried in air.
Classically, L-forms were isolated by cultivating cells in solutions containing high levels of chemicals that inhibit the synthesis of peptidoglycan, and lytic enzymes that break down cell walls (Allan, 1991).
