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The product (in gel form) was separated via centrifugation and washed with distilled water.
The monomeric form was separated from other higher oligomeric forms by glycerol gradient sedimentation.
The apo form was separated from the holo form using a Mono Q column pre-equilibrated with 10 mM Tris-HCl buffer (pH- 8.0) prior to the elution of the protein with a salt gradient (0 100% 10 mM Tris-HCl pH-8.0, 1 M NaCl).
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Consequently, the layers of halloysite in the hydrated form are separated by monomolecular water layers that are lost during dehydration.
The mixture system was stirred for 30 min. Then, the transparent solution formed was separated into two parts.
The precipitate which formed was separated from the solution by filtration, washed several times with distilled water and absolute ethanol, and then dried in air at 127C to obtain ZnO nanocrystalline powders.
The DNA-protein complex formed was separated from free oligonucleotide on 5% native polyacrylamide gel, and the gel was then dried.
The DNA-protein complex formed was separated from free oligonucleotide on 5% native polyacrylamide gel using buffer containing 50 mM Tris, 200 mM glycine (pH 8.5), and 1 mM EDTA, and the gel was then dried.
The DNA-protein complex thus formed was separated from free oligonucleotide on 7.5% native polyacrylamide gel, using buffer containing 50 mM Tris, 200 mM glycine (pH 8.5), and 1 mM EDTA.
The white precipitate formed was separated by filtration and washed with CH2Cl2.
The yellow precipitate that formed was separated by centrifugation and dried at room temperature.
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