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The mandibular form was quantified with 23 anatomical landmarks distributed approximately equally over the mandible (Fig. 2).
External body form was quantified by digitizing the Cartesian coordinates of 18 landmarks using TpsDig 2.10 (Rohlf, 2006).
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The blue color due to the complex formed was quantified spectrophotometrically at 820 nm.
The amount of formazan dye formed was quantified by its absorbance at 450 nm using a scanning multiwell spectrophotometer (MultiSkan MS, Thermo-LabSystems, France).
The number of neurospheres formed was quantified.
The amount of product formed was quantified as previously described.
The formazan dye formed was quantified by using a plate reader at 450 nm.
The [P]cAMP formed was quantified using a sequential chromatography technique.
Hydroxylated peptide formed was quantified by MALDI-TOF MS and succinate generated by LC MS.
Reactions were terminated, and the [P]cAMP formed was quantified using [H]cAMP added as a recovery marker.
The radioactivity of the [H]-fluorouridine monophosphate (FUMP) formed was quantified to determine the concentration of FUMP.
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