Env trimers of respective form were isolated from solubilized particles by sedimentation and their structures were reconstructed from electron microscopic images of both vitrified and negatively stained samples.
The dioxetane thus formed was isolated using column chromatography.
The resulting mixture was incubated several hours at 0 °C and the trioxacarcin DNA adduct formed was isolated by HPLC.
The solid diacid (3a) that formed was isolated via vacuum filtration, washed with water (3 × 200 mL), and dried overnight under vacuum at room temperature.
The solid polymer (4) that formed was isolated by vacuum filtration, washed with acidic water (100 mL) and dried overnight under vacuum at room temperature.
The membrane pellet formed was isolated and separated from other organelle membranes on a self forming Percoll gradient, and the mixture was centrifuged at 10 000 g for 30 min at 4°C.
After carbonization the MgO was dissolved out using a diluted sulfuric acid and the carbons formed were isolated.
After 7-days incubation at 25°C, colonies formed were isolated as alliin-utilizing strains, and were independently inoculated onto the agar plates for evaluation of odorous compound production.
The Bass Strait forms were isolated from Tasmania but more recently and so their subspecific status was not maintained.
Midgut forms were isolated from infected flies and fixed with 4% formaldehyde in order to preserve fluorescence.
