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Therefore, we examined the capability of three mesothelioma cell lines to form spheres under serum deprivation.
Therefore only the surviving fraction of growth factor-responsive sphere-forming cells divide, form spheres, and renew the founding population.
After the cells were allowed to form spheres for 20 days, sphere-forming capacity was analyzed by counting the spheres formed.
When sarcospheres were passaged to the third generation, U2OS/MTX300 cells had increased sarcosphere-forming ability, but U2OS cells had decreased ability to form spheres.
The stirring causes the nylon to form spheres that capture the nanotubes and the reactant.
The tube was cut into small sections that were rolled to form spheres, thereby making pills.
Like other cell types with self-renewing capacity, keratocytes can form spheres in culture.
This rotation, which the researchers call "CAMo," for coherent angular motion, is necessary for the cells to form spheres. Without CAMo, the cells do not form spheres, which can lead to random motion, loss of structure and malignancy.
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The resulting single cells were then centrifuged to remove the enzyme and re-suspended in serum-free medium allowed to re-form spheres.
CSCs can form spheres in the absence of serum under low adherence conditions.
He thought that he could prove his theory with a time-lapse photographic sequence of cells coming together to form spheres.
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