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Every known or predicted form of transcript of a gene will be assigned a systematic identifier that will be the same as the locus identifier except that the letter 'g' for gene will be replaced by letter 't' for transcript, to be added as a suffix following the two-digit chromosome identifier.
Further confirmation was in the form of transcript detection through use of RT-PCR with primers FL2194/FL2195 (Figure S1).
Recently, global analyses of mammalian transcriptomes suggest that alternative splicing is an important and prevalent form of transcript variation in many species [7].
Of these genes, 4783 had only a single form of transcript detected, and 1247 had two or more transcript isoforms.
Other high-throughput-based projects are delivering functional data in the form of transcript structure (Birney et al., 2007) and structural data is following a similar trend thanks to coordinated efforts like targetDB (Chandonia et al., 2006).
The "digital Northern", an in silico form of transcript profiling, can be used to study gene expression by comparing ESTs from clones randomly picked from two or more cDNA libraries created from non-normalized mRNA populations [ 3- 5].
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Although the nonfunctional form of transcripts accumulates under non-stress conditions, the functional form of transcripts is induced by abiotic stresses.
Qualitative data, in the form of transcripts, were interpreted using text-based content analysis.
Its rationale is that qualitative research produces large amounts of textual data in the form of transcripts, observational fieldnotes etc.
Data, in the form of transcripts and field notes, were analysed over a 4-month period using latent content analysis 24 25 and the constant comparative technique whereby subsequent codes within and across transcripts are compared and contrasted with previous codes.
These two forms of transcript analysis were run with independent samples (plants grown independently).
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