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The initial value was 0.5 for cells grown in liquid cultures and 0.9 for those grown in form of colonies.
Four lines had died on 9 January because of contamination, three were observed only in the form of colonies, not stem cell lines, and no records exist for two lines.
Author response: Although we agree that LECA lacked the features we associate with advanced multicellularity (i.e. cell diferentiation), we are not that sure that it was unicellular, as it may have lived in some form of colonies or consortia.
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Microorganisms were identified and numbered and results expressed in the form of colony forming units (CFU) per endoscope.
For the sake of concreteness, we may think of the growth dynamics of biological films formed of colonies of pseudomonas putida and listeria monocytogenes on a Petri dish with a substrate of nutrients.
Shankara was also responsible for the growth of Hindu monasticism, which had been in existence for more than a millennium in the form of hermit colonies.
The results of such an experiment are shown in the form of a colony formation assay in Figure 1C and quantitated in Figure 1D.
A typical screen consists of growing an array of strains on agar plates, forming a grid of colonies on each.
Csf-1rhigh cells and Csf-1rlow/− formed equivalent numbers of colonies when stimulated with multiple cytokines (Fig. 1e).
The 18Lin-CD34+ 18Lin-CD34+d all types of cellsies, including Cformed(61.5%), BFU-E (30.2%) all CFU-Mix (7.0%).
The true clonogenic/stem cells are capable of forming colonies of fibroblast-like cells at very low plating densities, and cell contacts would even inhibit cells growth.
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