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Like the MBP-fused chicken FST315, all four FST-type proteins were expressed in a soluble form (Data not shown).
Since it has been reported that native d-LDH from E. coli cells gives only a peak that corresponds to the molecular weight of 130 kDa (Tarmy and Kaplan [1968]), the apparently octameric form of ECLDH is likely an artificial product due to the overexpression, and exhibited slightly but significantly lower specific activity than the tetrameric form (data not shown).
However, under denaturing (but not reducing) solution conditions Alt a 1 effectively dissociated into its monomeric form (data not presented), providing evidence that its dimerization is not mediated by the disulfide bond.
The proteins were expressed well in soluble form (data not shown).
The affinity of recombinant HGF disappeared within one week after reconstitution of the lyophilised form (data not shown).
For five neurotransmitters, the protonated molecular ions were observed as the major ions, whereas norepinephrine could not be detected as the [M + H]+ ion form (data not shown).
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Two of our students commented on the value of formative assessment in the EVS lectures in free comments on their SELT forms (data not shown).
NMR analyses indicated that the commercial λ-carrageenan samples also contained κ- and ι-forms (data not shown), we therefore produced in-house cell wall extracts from C. crispus by separating the life-stages as a starting point (gametophytes only produced κ-/ι-carrageenans and sporophytes only produced λ-carrageenans)31.
When the thickness was over 10 nm, no gold nanoislands were formed, but a thin gold flat film was formed (data not shown).
We also tested a wide range of concentrations for all the antibiotics and observed that the Gnps conjugated antibiotics were more efficient than their respective free forms (data not shown).
However, we have found no antigenic evidence of other forms (data not shown).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com