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In checkpoint mutants, Rrm3 would remain unphosphorylated and active, generating a fork configuration opposite the one of WT cell, in which the lagging strand would be more elongated than the leading one.
This asymmetric stalled fork configuration with advanced leading strand and stable replisome- or helicase-fork complexes may facilitate, in some way, fork restart following HU removal and checkpoint deactivation.
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Callaghan [ 16] studied the molecular motion of tristearin in the melt and found different T2s along the chains, which in turn were used to explain the tuning fork molecular configuration.
At this point you can customize everything you want, but effectively you are forking our configuration and going your own way".
Our findings suggest that in WT cells, the leading protruding configuration of stalled forks would protect them from fork reversal.
We analyse the performances of two different configurations of a tuning fork microgyrometer, the so called 'wide gap' design and 'narrow gap' design.
Puppet [14] is a configuration management system originally forked from CFEngine.
A combination of plugin configuration and the ability to fork an existing plugin means that it is possible to create bespoke behaviour easily.
Just like the varied ways the wooden spoons and forks are displayed in homes, "Spoon and Fork" is meant to be hung in any configuration, Gaspay explained.
While in a practical situation, cracks show more complex configurations, such as "chevron", "branch" and "fork" [73].
One possibility is that Fob1, by obstructing the migration of the fork branching point, has been evolutionarily selected to avoid a fork pausing configuration dependent on the checkpoint.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com