Therefore, in addition to targeted gene sequencing, brute force sequencing of entire shared regions may be appropriate.
These approaches typically involve some form of microsatellite enrichment, followed by time consuming and costly brute force sequencing.
In particular, brute force sequencing initiatives will result in a better mapping of the functionally important regions in the genome and experimental approaches, such as CRISPR/Cas [ 128] will provide the opportunity to extensively test the functional impact of genetic variants.
While germline mutations in KCNJ5 and CACNA1D were discovered following the initial identification of their somatic mutations in APAs (Choi et al., 2011; Scholl et al., 2013), the discovery of the recurrent CACNA1H mutation relied entirely on brute force sequencing of patients with early severe aldosteronism and hypertension.
For as long as teams have played football, the goal-line stand has proved to be a game-changer, a brute-force sequence, man against man, power against power, strength against strength, greatly shifting momentum from a team that is about to score to a team that refuses to yield points.
The special forces sequence was the centrepiece, and pretty much every other piece, too – it dragged on and on and on.
That hand is a tad strong for a game-invitational sequence and a tad weak for a game-forcing sequence.
Richard Gibbs and colleagues at the genome center at Baylor College of Medicine in Houston, Texas, will lead the effort, and a group at Celera Genomics in Rockville, Maryland, will add brute-force sequencing power.
The novel feature is the use of information generated on the previous trial to compute the control input for the next one and the basic problem is to force the sequence of trial outputs to track a given reference signal.
