Optical force probe assays show that the PCM of rat chondrocyte joint (RCJ-P) cells easily reconfigures around optically manipulated microparticles, allowing the probes to penetrate into rather than compress the matrix.
For forced proliferation assays, two GAL4 lines were used: nanos-GAL4VP16 was used to achieve overexpression in the germline with UAS constructs for RasV12, CyclinE, and Thickveins (Rorth, 1998).
Later on, Zhu and his colleagues used single-molecule biomechanical force-clamp assays to demonstrate catch bond formed between α5 β1, α L β2, and α4 β1 integrin with their respective ligands [ 64, 66– 66].
To investigate how specific the interaction between the Ebola fusion peptide and microdomains is, we have carried out single molecule force spectroscopy assays.
While repeated non-invasive interrogation of myobundle function was limited to calcium imaging, integration of smaller size myobundles with high-throughput force testing assays should be feasible as demonstrated for mouse cells (Vandenburgh et al., 2008).
DA planned the atomic force microscopy and cell toxicity assays and participated in preparing the manuscript.
As we will describe in detail in this section, various single-molecule approaches, such as single-molecule fluorescence-based methods, atomic force microscopy, and magnetic tweezers assays, have provided a detailed picture of transcription initiation.
When you start getting into psychiatry, we have tail suspension assays, we have forced swim assays, we have learned helplessness assays... none of which have been developed through a detailed understanding of the pathophysiology.
(ii) An aging-related slowing of motility speed was observed in both type I and IIa MyHC assays, but the force-generating capacity was unaffected by age, irrespective of MyHC isoform.
In dual-choice feeding-preference and force-feeding assays, the transgenic tobacco lines expressing GmTPS3 exhibited enhanced resistance to cotton leafworms and an increased level of geraniol.
