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Understanding the camel forage array is of utmost importance for the growing sphere of camel herders in Karamoja.
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Using loop design [ 32], each Forage Quality Array was hybridized with two contrasting lines (Table 1) labeled with different dyes.
According to the above mentioned criteria, 865 different candidate ESTs were identified towards production of the Forage Quality Array.
In order to verify the most interesting of those genes at low costs, a small microarray specific for cell wall digestibility (Forage Quality Array) was designed.
23% of the ESTs on the Forage Quality Array (102 of 439) were significantly different between low and high digestible (dNDF) RILs.
Out of those, 439 ESTs were assembled on our "Forage Quality Array", a small microarray specific for cell wall digestibility related experiments.
Transcript profiles of 40 lines of a Flint × Flint population were monitored using the Forage Quality Array, which were contrasting for cell wall digestibility.
Using hierarchical cluster analysis based on the EST tree, distinct expression patterns between high and low quality groups were found for most ESTs included on the Forage Quality Array.
The objectives of our study were to 1) select candidate ESTs for cell-wall digestibility to establish a "Forage Quality Array", 2) identify ESTs differentially expressed between low and high digestible lines in a Flint × Flint mapping population, and 3) detect eQTL using the "genetical genomics" approach.
AU carried out the hybridization of Forage Quality Arrays.
In total, 40 Forage Quality Arrays were used and each line was employed with dye-swap replication.
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