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Using these culture conditions, we and others found that HME cells in general, and MCF10A cells in particular, have an absolute requirement for insulin and EGF for continuous growth in serum-free media.
(a) Seeds were sown in triplicate on standard medium with different concentrations of mannitol (0 300 mM) for continuous growth for 5 days.
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Mechanisms associated with screw dislocations, or twinning edges, can provide for a continuous growth of crystals.
This helps for the continuous growth of the nanofibers with successive laser pulses.
The first three are seen as essential for the initial growth of the sector, while the last three are seen as essential for the continuous growth.
Then, PDA coating was used as an active support for the continuous growth of Au nanoclusters (Au NCs), by CV method using optimized growth parameters.
Subsequent twinning within the twinned tetrahedrons provides additional re-entrant grooves on their top faces, which are important for the rapid elongation and consequently for the continuous growth of the branched particle.
Results show that while the emergence of critical Reynolds number regime is crucial for the onset of dry inclined cable galloping, the spanwise correlation of aerodynamic forces acting on a cable and the sustained duration of critical flow condition are the determining factors for the continuous growth of unstable cable response and the eventual occurrence of this type of aerodynamic instability.
Kienast and coworkers observed that brain metastasis initially proliferating by co-opting vessels needed remodeling of the existing vasculature for the continuous growth of micrometastasis.
The microtubule motors kinesin and dynein cooperatively deliver secretory vesicles via microtubules and are likely to be important for maintaining continuous growth (Steinberg 2011).
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CEO of Professional Science Editing for Scientists @ prosciediting.com