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However, the precise control of genetic regulation is poorly understood for whole cells and large systems.
Isolation of enzymes, either in a crude or purified form, permits biotransformation directly without the need for whole cells.
The affinity of dansyl[Lys]1polymyxin B3 for LPS was comparable to polymyxin B and colistin, and considerably greater (Kd < 1 μM) than for whole cells (Kd ∼ 6 12 μM).
While isolated membranes are generally well-immobilized by simple adsorption on mica [27], stronger attachment is often needed for whole cells [28].
However, it can be assumed that due to the high complexity and the presence of more sensitive molecules, the effective treatment time for whole cells is situated at a shorter exposure time.
After incubation in the sulfuric acid with slow rotation at 4°C for 2 h, proteins were treated the same way as described for whole cells above.
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In this study we present a theoretical framework and an algorithm for whole cell tracking.
We describe methods for whole cell ultrastructural imaging that can provide three-dimensional views of intraerythrocytic development.
In particular we demonstrate the potential of electron tomography for whole cell studies to enable 3-D distributions of particles within cells.
This is consistent with the experiments, and an alternative method is needed if the LSPR sensor is used for whole cell bacteria detection.
This proof of concept study indicates that the lateral flow assay using engineered CBDs hold considerable promise as simple, rapid, and cost-effective biosensors for whole cell detection.
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