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Exact(6)
Footpaths, guttering, sewerage, landfill – issues that were overcome decades ago in normal terrestrial environments – represent a complex set of problems in countries like the Marshall Islands, Kiribati and Tuvalu, for which viable solutions have yet to be found.
This approach does require that mediators in question operate through a receptor for which viable, reproductively normal, receptor-deficient mice exist.
Our gene profiling experiments included every sample for which viable tumor tissue was available to establish at least short-term cell cultures (N = 10, Table 1).
These numbers include only KO lines for which viable mice survived through 14 weeks of age.
Arabidopsis thaliana is the only higher eukaryote for which viable CAF-1 mutants are available (for review see [ 34]).
Although we analyzed only a subset of miRNAs for which viable overexpression lines could be obtained, it is reasonable to assume that these observations would extend to the larger ensemble of miRNAs.
Similar(54)
However, resistant strains might have gone undetected, as an estimated 97% of genital gonorrhea testing in Michigan is performed by nucleic acid testing, from which viable isolates for susceptibility testing cannot be obtained (MDCH, unpub. data).
The plates were incubated in a 37°C CO2 incubator for 21 days after which viable colonies were stained for 24 hrs with 200 mL of 1 mg/mL nitroblue tetrazolium.
If immunogenicity is determined to be problematic, the use of appropriate immune-suppressive treatment for the time during which viable cells are present in vivo would circumvent immune-related limitations of the described approach.
DBA/2 CD4+ T cells were cultured with 20 Gy irradiated BDF1 DCs in the presence or absence of HGF for 3 days, after which viable cells were cultured with BDF1 B cells and MHC class II expression assessed.
TC-32.Fb-neo and TC-32MRP-1.Fb-neo cells were treated with increasing concentrations of the MRP-1 substrates doxorubicin (0.85 56 n), vincristine (2 120 n), etoposide (7 240 n) and a non MRP-1 substrate, actinomycin D (0.1 7.2 n) for 48 h, after which viable cell number was counted using the trypan blue exclusion assay (Vi-cell, Beckman Coulter, High Wicombe, UK) (Myatt et al, 2005).
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