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For the age of death, we found no effect of exposure, except for GG3 individuals exposed to high dose of Sp1, for which lifespan is shorter than controls (F = 3.53, P = 0.019; Figure 1C).
Table 3 contains results for the larger subset of points for which lifespan and body mass measurements were available (i.e., including those pairs lacking fecundity or generation time data).
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Finally, we also tested the two mutants pka1 and git3, for which no lifespan scores could be computed but that exhibit extended lifespan with limiting glucose during stationary phase (Roux et al. 2006).
The males for which adult lifespan was assessed had been tested in a wind tunnel just after emergence (see below).
For these studies, we required an organism with relatively short lifespan that could be assayed reproducibly and robustly, and for which the genetic and environmental factors affecting lifespan were well defined.
As such, LIFEspan does set the stage for an ideal model for which to strive in service delivery, where, for example, LIFEspan clients benefit from a publicly funded health care system and transportation services to and from the centre.
All the analyses for adult lifespan were performed on natural log transformed values of the individual lifespan data, since the adult lifespan data have a long right-hand tail for which mean value is not an appropriate measure for comparison.
Maximum lifespan was calculated as the shortest survival time for which the survivor function is ≤0.95.
One-way ANOVA followed by Dunnett's multiple comparison or an unpaired two-tailed t-test was used to test for significance except the lifespan curves, which were compared using Log-rank (Mantel Cox) tests and the ChIP experiment, for which a paired t-test (within the same experiment) was used.
Recent work suggests that sirtuins can modulate ROS levels notably during a dietary regimen known as calorie restriction which enhances lifespan for several organisms.
These observations provide a foundation for future functional studies in which the lifespan of a worm may help us to understand type II diabetic insulin resistance.
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