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For virus inoculation experiments, Ae. aegypti Aa20 were seeded at the density of 3 × 105 cells per well in 12-well plates.
T3 homozygous lines were used for virus inoculation.
For virus inoculation, confluent cells were trypsinized and diluted 1 4 in medium and 2.0 ml was added to six-well microplate (Corning, USA) 1 or 2 days before virus infection.
For virus inoculation, 6 cats were anesthetized with xylazine hydrochloride (30 mg/kg intraperitoneally), after which they were inoculated with virus (10 TCID50 [median tissue culture infective dose]) in 1.0 mL of phosphate-buffered saline (0.5 mL in each nostril).
This consisted of the supernatant of MDCK cells that were treated in an identical manner (except for virus inoculation) to those that were used to prepare the virus stock.
For virus inoculation, Micro-Tom plants were grown in a mixture of vermiculite and perlite (1 1 (v/v)) at 23-25°C 23-25°Cphotoperiod of 16 h light (8000 lux, or 97 μmol/m2 s)/8 h dark, and irrigated with a nutrient medium every three days.
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For virus inoculations, TMV solutions of 0.1 to 0.5 mg/ml were rub-inoculated onto carborundum-dusted leaves.
Three-week-old Nicotiana benthamiana plants and nine day old zucchini squash (Cucurbita pepo L. cv. Green Bush) plants were used for virus inoculations or agroinfiltration.
Animals in both the ZIKV-treated and placebo cohorts were sedated at time zero (time of virus inoculation), days 2, 3, 5, and 7, and then weekly for sample collection and ultrasound monitoring.
The method of virus inoculation requires careful consideration in the design of ferret experiments as a model for influenza A/H5N1 in humans.
Intranasal administration of virus in a liquid suspension has long been the traditional method for influenza virus inoculation in the ferret model.
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