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Data for viable counts of the microorganisms were normalized by logarithmic transformation, and nontransformed values were presented in the Results.
To estimate the amount of intracellular bacteria at each time point, cells were lysed using 1% Triton X-100 (Sigma), and samples were taken for viable counts [10].
To determine the levels of intracellular bacteria at each time point, cells were lysed using 0.1% SDS, and samples were taken for viable counts [10].
Cough aerosol produced by patients colonized with MDROs was measured for viable counts.
Carriage was not accompanied by bacteremia as determined by plating blood samples obtained by cardiac puncture for viable counts.
Bacterial suspensions were heated at 65 , 75and 80 °C and samples for viable counts were obtained after 1,2,3 and 10 minutes at each temperature.
Similar(49)
Immediately after inoculation 100 μl was collected from the solutions for viable count.
Weights of tissues were determined for the homogenized samples to provide a denominator for viable count and cytokine level data.
The OD600 was recorded over 72 h and 100 µl samples were taken periodically and stored at 4°C for viable count analysis.
Samples for viable counting were withdrawn at 0, 2, 4, 6, 8, and 24 h.
Samples were diluted in cold 10-2 MgSO400ontaining 400 U/ml catalase, followed by plating on LB plates for viable count.
More suggestions(15)
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