Exact(29)
Since nonadherent dead cells are removed during the washing process of this assay, cells observed as deep blue, stained by crystal violet, account for viable cells only.
Protective pore spaces in the pumice stone carrier employed in 2015 may have also functioned at the same time as a slow-release tool for viable cells to be progressively supplied to plant roots.
Geologist L. Paul Knauth of Arizona State University in Tempe adds that Earth's early oceans were up to twice as salty as they are today--making it even more difficult for viable cells to arise.
Upon elimination of cell debris according to size and granularity, events that were positive for SCA-1 and CD90.2 antibodies were collected for viable cells (Fig. 2).
For each trial, 50 µl of Jurkat T cells were mixed with 50 µl of 4% trypan blue; 20 µl of the cell suspensions were enumerated for viable cells by hemocytometer counts (Figure 1E and S1).
The cultures were allowed to grow at 30°C for 5 days, then a small amount diluted 105−fold on YPD to assay for viable cells, and the remainder concentrated to 1 mL, and 100ul plated on 0.04% Canavanine-containing synthetic complete medium to assay for CanR mutants.
Similar(31)
For the quantitative measurement of biofilm-embedded microbial cells, a culture-based method for viable cell count was used.
Then, 1 mL of the tested suspension sample was collected every 1 h for viable cell counting in MHA plate followed by incubation at 37 °C for 24 h.
The changes induced by the presence of nanoparticles in the C. albicans biofilm formation on textile dressing samples were assessed by quantitative, culture-based methods for viable cell counts (VCCs) assay, and qualitative analysis was performed by scanning electron microscopy (SEM).
Samples were periodically withdrawn and plated on rich medium for viable cell counts (Methods).
After brief centrifugation at 1,000 r.p.m for 5 minutes to remove dead cell debris and the low-density stromal cells, the cell pellets were resuspended for viable cell counting using 0.4% trypan blue.
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