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The individual sections were then frozen and then analyzed visually for viability with preservation of orientation.
Plates were incubated at 37°C for 6 h and then stained for viability with the amine-binding dye Aqua (Invitrogen) and then washed.
The cells of the interphase were washed twice, counted and tested for viability with trypan blue dye.
Lymphocytes for each donor were pooled, counted, assessed for viability with trypan blue (>98% in all cases), frozen and stored in liquid nitrogen.
Alternatively, cells were counted and tested for viability with Trypan blue after trypsinization, and plated under non-attachment conditions with mammosphere medium.
Bcl-2-overexpressing cells and control cells were plated 24 h later and tested for viability with a trypan blue exclusion assay as described above.
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A Drosophila homolog of these proteins, without children (dWoc), is essential for viability, associates with chromatin, and prevents telomere fusions [30] [32].
Because cell size is important for viability, mating with a partner of the right size could be a significant advantage.
In cardiology, [F]FDG has been approved for viability assessment with PET by FDA and can be considered the gold standard for detection of the reversibility of injured myocardium in the clinical setting [ 89].
One probiotic strain, L. reuteri NCIMB 701089 (P < 0.05, subset "a"), demonstrated the most assimilation, when cholesterol assimilation is normalized for viability counts, with 2254.70 ± 63.33 mg of cholesterol assimilated per 10 cells.
GRFs are multifunctional and essential for viability, consistent with their role in enabling the function of numerous other TFs and/or in chromatin opening at numerous promoters (Badis et al. 2008), presumably including those of other essential genes.
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