Sentence examples for for viability counts from inspiring English sources
For viability counts, samples were diluted in 0.9% NaCl, spread or spotted on LB plates and incubated 12-16 hours before colony counts were taken.
One probiotic strain, L. reuteri NCIMB 701089 (P < 0.05, subset "a"), demonstrated the most assimilation, when cholesterol assimilation is normalized for viability counts, with 2254.70 ± 63.33 mg of cholesterol assimilated per 10 cells.
Indeed, when normalized for viability counts, one probiotic strain, L. plantarum ATCC 14917, assimilated the most cholesterol, with 15.18 ± 0.55 mg of cholesterol assimilated per 10 cells (P < 0.05, subset "a").
The PAE was defined according to the formula: PAE (in hours) = T- C, where T is the time required for viability counts of an antibiotic-exposed culture to increase by 1 log unit above counts taken immediately after dilution and C is the corresponding time for the growth control, as previously described [ 1].
For viability counting, dead embryos were characterized by the presence of large, dark debris in the embryos.
For performing embryonic viability counts, we transferred >10 L4 hermaphrodites to individual plates and grew them at the permissive (15°C) or restrictive (26°C) temperatures until broods were produced.
For each experiment, viability counts (log CFU/mL) were plotted against time and expressed as the means of results from three separate assays.
The PAE was defined according to Craig and Gudmundsson [Craig] as PAE = T − C, where T is the time (in h) required for the viability count in the test culture to increase 1 log10 above the count observed immediately after centrifugation, and C is the corresponding time for the controls.
Viability counts were determined before exposure, immediately after centrifugation (0 h), and then hourly for 5 h by plate counting.
Concurrent to removing test pegs for the indirect qualitative outgrowth test, replicate sample pegs were removed and directly examined for viability by the quantitative viability counting method and direct qualitative outgrowth test for comparison.
For cell viability assay, 10 μl of suspension culture was taken in eppendorf tube, added 190 μl of via count solution (viability counting solution) and incubated for 5 min at room temperature.
