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Our research has provided novel insight into cell division and morphogenesis, processes essential for viability and development, and defective in human diseases such as cancer.
The C. elegans KASH domain protein KDP-1, in conjunction with SUN-1, regulates cell-cycle progression in the germline and in early embryos, and is essential for viability and development (McGee et al. 2009).
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In the present study, the zebrafish Danio rerio was used to investigate whether Naa10 is necessary for viability and early development in a vertebrate.
Our results indicate that Naa10 is essential for viability and normal development in zebrafish, with knockdown resulting in severe and reproducible phenotypes, including generalized malformations and short body length.
LID is critical for Drosophila viability and development (Gildea et al. 2000; Li et al. 2010).
To determine whether Naa10 is essential for zebrafish viability and development, we targeted embryos with naa10-specific splice blocking MO [ 1].
Panicles from the crosses, TH4n × TH4n, Nip2n × Nip2n, Nip2n × TH4n (paternal excess), and TH4n × Nip2n (maternal excess) were harvested for analysis of seed set, viability and development.
When we examined deletion alleles of tba-1, tbb-1 and tbb-2 generated by the C. elegans Gene Knock-out Consortium for viability and defects in D neuron development we found similar results.
This "traditional approach" can increase considerably risks for the product and can have negative consequences in product viability and development costs.
We conclude that BAF and PBAF genes are required for viability and specific subunits appear to play distinct roles in early development.
ego-1 is essential for viability and fertility and in particular plays a crucial role in germline development, where it promotes cell proliferation, meiosis, and gametogenesis.
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