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Here, we employed SRM assay for verification of SILAC data, since we have previously validated its accuracy and effectiveness for verification of candidates in amniotic fluid [ 15].
The previously reported Affymetrix SNParray data [ 15] was used for verification of our BACarray results on tumors common to both studies (n=15) and for verification of our data on presently unique tumors (n=19) new original SNParray data was produced.
The participants were contacted for verification of analysed data from the full interview transcript and the summary.
Completed surveys were mailed back to the Mayo Clinic Survey Research Center for verification of consent and data entry.
A number of differentially expressed genes were selected for verification of the microarray data by semiquantitative PCR.
For verification of array CGH data by FISH we used a set of BAC clones mapping to 15q24.
For verification of the array data, 4 genes out of the 73 potential biomarkers were subjected to qPCR.
The medical records and other documents will be reviewed for verification of agreement with data on the CRF.
This gives hope that high-throughput proteomics techniques can be used to improve and validate gene annotations, and in particular for verification of high-throughput, automatic gene annotations.
Second, the U.S. EPA (2011) guidelines discuss access to raw data as important for verification of conclusions.
Collection of data at specified points for verification of the land use maps.
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