Cells were pre-incubated with cycloheximide and stimulated with LTD4 for various time points.
Hexon-modified vectors, as well as controls vectors, were subjected to heating at 45°C for various time points (0,5,10, 20 and 45 minutes).
The irradiated cells were incubated at 37°C for various time points, and fixed and processed for immunofluoresence staining with Alexa 488-Phalloidin.
To investigate attachment and subsequent proliferation of keratinocytes, an adhesion assay [25] was carried out, where cells were allowed to adhere for various time points following plating.
For this, BMM from wild type and Myr Akt mice were infected with F. tularensis subsp. novicida for various time points.
Fas protein expression was verified by Western blotting protein-matched lysates from BMM infected with F. tularensis subsp. novicida for various time points.
Because in our country the processing practice for colectomy specimens does not include buffered formalin, tissue fixation had been accomplished in the majority of cases in simple formalin (∼4% formaldehyde) for various time points.
In this context, KG-1 cells were stimulated with rIL-1β, rIL-18 and rTNFα for various time points, with and without co-addition of IL-1ra or IL-18 Ab.
Subconfluent VSMCs were growth-arrested by serum deprivation for 24 h and subsequently stimulated with recombinant profilin-1 (up to 1 µM) for various time points in the presence or absence of pharmacological inhibitors as indicated.
We investigated the activation of several signaling pathways in macrophages in response to antibody-coated tumor targets by stimulating IFNγ-primed peritoneal macrophages with formaldehyde-fixed Rituximab-coated Raji targets for various time points.
We also synchronized primary human foreskin fibroblasts in G0 through a physiological process, i.e., contact inhibition, followed by release for various time points to allow progression through the cell cycle (Fig. 3B).
