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To help design the assembly primers, as well as to optimize codon usage for various expression systems, some dedicated computer software [19] and internet online tools [12], [20] have been developed.
The differences between the functional networks are better described by considering the main hubs or central nodes which were common for various expression types and those which appeared only in particular conditions.
Therefore, while follow-up work is needed to confirm expression levels, examination of ESTs provides a list of candidates for various expression profiles.
This system allows the visualisation of strongly concordant or discordant results for subsets of genes selected for various expression fold changes and p values.
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Lexical features here refer to language related information regarding the fact that people tend to choose specific words for various expressions (e.g., "can't", "damn", "bad", and "great") [9].
For GO analysis of various expression profiles, we applied the annotations of "Biological Process" (BP) domain and the minimum expression fold change (in log2 scale) was set to different values from zero.
A purity of 95% or higher was found for all proteins even of various expression attempts of same protein.
Looking first at the results for total (soluble and insoluble) expression, no clear patterns emerge for the various expression vectors used.
Our results indicate, however, that the flow of information in the integration mechanism is different for the various expression types.
This indeed calls for precaution in evaluating various expression systems.
They also receive ongoing training and support for the various expressions of emotional distress and crisis management.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com