Importantly, the experimental design provides internal, paired control fibrils for each experiment (fibrils not strained between the pipettes), which removes complications resulting from variability in the preparation.
Each peptide series (varying at one position) was analyzed in parallel to account for variability in enzyme activity between preparations and during storage.
We focused primarily on commercial assays because of the less variability in preparation of reagents and standards, which enables generation of more accurate and precise results.
However, the fact that quite distinct differential effects could be elicited in individual preparations suggests that methodology was not the primary reason for the variability in the relative positions of chronotropic and dromotropic maxima.
Nevertheless, there is a lack of uniform guidelines for PCC administration [9, 10, 18, 19], considerable variation in practice among clinicians [32] as well as great variability in the composition of PCC preparations [2].
For all the variability in the meaning of marriage, one fairly consistent element over time and place was that it had nothing to do with love.
The preparation of homogeneous, and ideally clonal, populations would be expected to minimize variability and therefore be desirable if not essential for many quantitative or high throughput applications.
Our results show that approximately 80%and32%2% of the variability in the changes of teat canal length from pre-teat preparation to 0 and 20 minutes after milking, respectively, could be explained by changes occurring during teat preparation.
We note that some variability in the detection of the oxyanion peaks, as illustrated by a comparison of representative difference sample preparations in Fig. S5.
Each peptide series (varying at one position) was tested in parallel to account for possible variability in the reactions due to changes in enzyme activity upon storage and from preparation to preparation.
